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US 8,895,273 B2Granted
Conversion of hexuronic acid to hexaric acid
AssigneeTeknologian tutkimuskeskus VTT Oy
InventorsHarry Boer, Satu Hildich, Peter Richard, Merja Penttila
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AssigneeTeknologian tutkimuskeskus VTT Oy
InventorsHarry Boer, Satu Hildich, Peter Richard, Merja Penttila
Estimating the term…
The present invention relates to a method and microbial host strain for converting a hexuronic acid to a hexaric acid. In particular, the invention relates to the conversion of D-galacturonic acid to meso-galactaric acid (mucic acid). The invention also concerns an isolated nucleotide sequence. According to the present method a microbial host strain genetically modified to express uronate dehydrogenase enzyme (EC 1.1.1.203) is contacted with a biomaterial comprising hexuronic acid and the conversion products are recovered. By using the recombinant microorganisms of the present invention it is possible to treat biomaterials comprising hexuronic acids and thereby decrease the amount of hexuronic acids released to the environment.
A method for converting galacturonic acid to galactaric acid, said method comprising contacting a eukaryotic microbial host strain genetically modified to express uronate dehydrogenase enzyme (EC 1.1.1.203) comprising an amino acid sequence according to SEQ ID NO:4 with a biomaterial comprising galacturonic acid and recovering conversion products comprising galactaric acid.
A method for treating biomaterial comprising galacturonic acid, said method comprising a step of a eucaryotic microbial host strain genetically modified to express uronate dehydrogenase enzyme (EC 1.1.1.203) comprising an amino acid sequence according to SEQ ID NO:4 being contacted with said biomaterial under suitable culture conditions.
A genetically modified eukaryotic microbial host strain expressing uronate dehydrogenase enzyme (EC 1.1.1.203) comprising amino acid sequence according to SEQ ID NO:4 and converting galacturonic acid to galactaric acid.
A genetically modified fungal host strain transformed with a bacterial nucleotide sequence encoding a protein having uronate dehydrogenase enzyme activity (EC 1.1.1.203) comprising amino acid sequence according to SEQ ID NO:4 and said host strain converting galacturonic acid to galactaric acid.
Dependencies are inferred from the claim text.