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US 8,895,268 B2Granted
Preservation of information related to genomic DNA methylation
AssigneeIllumina, Inc.
InventorHenri A. Kester
Life of this patent
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Abstract
The present invention relates to compositions, methods and systems for analyzing the methylation state of nucleic acids. Some embodiments relate to a compositions, methods and systems for analyzing the methylation state of DNA with a gene array.
Claims20
An isolated nucleic acid pair comprising:
- a template nucleic acid comprising a cytosine residue;
- a complementary copy of said template nucleic acid having every cytosine replaced by a bisulfite-resistant cytosine analog;
- and
- a tag capable of identifying said template nucleic acid and said complementary copy of said template nucleic acid as members of said nucleic acid pair, wherein said template nucleic acid and said complementary copy of said template nucleic acid are coupled to said tag.
1 more element
A population of isolated nucleic acid pairs, comprising:
- template nucleic acids and complementary copies of said template nucleic acids attached to each other as covalent pairs via a nucleic acid loop,
- wherein said template nucleic acids each comprise at least one cytosine residue,
- wherein every cytosine of said complementary copies is replaced by a bisulfite-resistant cytosine analog;
- and
- wherein different nucleic acid pairs in said population have different sequences for said template nucleic acids and the same sequence for said nucleic acid loop.
2 more elements
An array comprising:
- a solid support with a plurality of sites,
- a bisulfite-converted template nucleic acid, and
- a non-converted complementary copy of said template nucleic acid,
- wherein said template nucleic acid is coupled to at least one of said plurality of sites and said non-converted complementary copy is coupled to at least one of said plurality of sites.
1 more element
Dependencies are inferred from the claim text.