Staining method for identification of flatworms

Abstract

The staining method for identification of flatworms includes placing a sample flatworm specimen on a microscope slide, mounting the flatworm specimen on the slide with a small amount of lactophenol cotton blue (LPCB) stain, waiting two to three minutes for the stain to be absorbed by flatworm tissues, covering the specimen with a cover slip, and examining the stained specimen with a microscope to identify the species-distinctive organs of the flatworm. For permanent mounting, the cover slip may be sealed to the microscope slide by nail polish. The method is effective for trematodes (flukes) and cestodes (tapeworms). Before examination, the specimen may be preserved by obtaining the flatworm from a carrier or host, washing the flatworm in saline and preserving the flatworm in alcohol, e.g. 70% alcohol.

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