Use of fluorescent saccharide-based derivative
AssigneeI-Shou University
InventorsJau-Yann Wu, Li-Feng Liu, Kuan-Ju Pan, Hao-Yu Tseng, Jian-Ching Wu
Life of this patent
Estimating the term…
Abstract
The disclosure provides a use of a fluorescent saccharide-based derivative in cell detection. The fluorescent saccharide-based derivative is obtained from a reactive material through an imine formation reaction, and the reactive material is selected from a composition (A) or a composition (B). The composition (A) includes a reducing sugar compound and an amino group-containing compound having at least one primary amino group. The composition (B) includes an amino sugar compound and a carbonyl group-containing compound, and the amino sugar compound contains at least one primary amino group.
Claims11
A method of detecting cells using a fluorescent saccharide-based derivative, including:
- incubating at least one sample with a fluorescent saccharide-based derivative;
- emitting an excitation light to the at least one sample;
- and
- detecting fluorescence emitted from the at least one sample,
- wherein the fluorescent saccharide-based derivative is obtained from a reactive material through an imine formation reaction, and the reactive material is selected from a composition (A) or a composition (B):
- the composition (A) comprises a reducing sugar compound and an amino group-containing compound having at least one primary amino group;
- the composition (B) comprises an amino sugar compound and a carbonyl group-containing compound, wherein the amino sugar compound has at least one primary amino group.
Dependencies are inferred from the claim text.