Method for determination of platelet function under flow conditions

A method for the determination of an antithrombotic effect of a P2Y(12) antagonist or a P2Y(1) antagonist in a whole blood sample, the method comprising the following steps:

• a) passing the blood through a capillary and then through an opening of a partition member which comprises at least one platelet activator from the group of purinergic receptor activators and which further comprises collagen;
• and
• b) measuring the time that is required for the formation of a thrombus at the opening of the partition member up to closure of the opening;
• wherein the whole blood sample is mixed with forskolin in a final concentration of from 0.1 to 5 μM before passing through the capillary,
• wherein the whole blood sample comprises one or more of the P2Y(12) antagonist and the P2Y(1) antagonist, and optionally an acetylsalicylic acid, and
• wherein the time measured in step (b) indicates the antithrombotic effect of the P2Y(12) antagonist or the P2Y(1) antagonist in the whole blood sample, and wherein the time measured in step (b) does not indicate the antithrombotic effect of the acetylsalicylic acid in the whole blood sample.