Device for measuring proteins using biosensor
AssigneeI-Sens, Inc.
InventorsHakhyun Nam, Su-Moon Park, Jin-Young Park, Joo Young Cho
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Abstract
A sensor for measuring a concentration of a specific protein using a biosensor with a measurement speed improved from a conventional impedance measurement. The sensor is capable of efficiently and accurately measuring impedance generated by a selective binding to the protein by Fourier-transforming an electric current signal obtained by applying a potential signal of a delta function waveform. The device for measuring a protein using a biosensor is capable of measuring concentration of the protein with accuracy, measurement time is shortened and the concentration of protein can be accurately measured by removing the influence of dispersion.
Claims13
A method of measuring a concentration of a specific protein using a biosensor comprising:
- feeding a sample comprising the specific protein into a biosensor;
- wherein the biosensor comprises a sample inlet through which the sample is drawn in, a working electrode on which a receptor layer is coated for selective binding to the specific protein in the drawn sample, and a measuring unit comprising a reference electrode to form a potential difference with the working electrode,
- applying a potential signal in a form of delta function waveform to the working electrode and the reference electrode;
- and
- measuring an impedance of the working electrode by Fourier-transforming an electric current obtained in response to the delta function waveform,
- wherein the specific protein is a glycated protein, and
- the receptor layer is formed as a self-assembled monolayer (SAM) which have boronic acid derivative as an end group.
A method of measuring a concentration of a specific protein using a biosensor comprising:
- feeding a sample comprising the specific protein into a biosensor;
- wherein the biosensor comprises a sample inlet through which the sample is drawn in, a working electrode on which a receptor layer is coated for selective binding to the specific protein in the drawn sample, and a measuring unit comprising a reference electrode to form a potential difference with the working electrode,
- applying a potential signal in a form of delta function waveform to the working electrode and the reference electrode;
- and
- measuring an impedance of the working electrode by Fourier-transforming an electric current obtained in response to the delta function waveform,
- wherein the specific protein is a glycated protein, and, the receptor layer is formed as a self-assembled monolayer (SAM) which have boronic acid derivative as an end group, and the boronic acid derivative is partially transformed into a thiol group to easily combine with the working electrode.
A method of measuring a concentration of a specific protein using a biosensor consisting essentially of:
- feeding a sample comprising the specific protein into a biosensor;
- wherein the biosensor comprises a sample inlet through which the sample is drawn in, a working electrode on which a receptor layer is coated for selective binding to the specific protein in the drawn sample, and a measuring unit comprising a reference electrode to form a potential difference with the working electrode,
- applying a potential signal in a form of delta function waveform to the working electrode and the reference electrode;
- and
- measuring an impedance of the working electrode by Fourier-transforming an electric current obtained in response to the delta function waveform,
- wherein the specific protein is a glycated protein, and the receptor layer is formed as a self-assembled monolayer (SAM) which have boronic acid derivative as an end group.
Dependencies are inferred from the claim text.