US 8,895,248 B1 · Method for detecting Salmonella species by assaying outer membrane Porin F (ompF)

Abstract

The invention relates to a method of detecting the presence of Salmonella in a sample using novel oligonucleotide sequences. Also presented is a kit for putting the method into practice and novel nucleic acid sequences for ompF. The ompF gene was found to be 100% inclusive for Salmonella species and 100% exclusive for non-Salmonella species for the strains tested thus making it an excellent marker for identification of both the species of Salmonella: S. enterica and S. bongori. Two hundred and eighteen isolates belonging to Salmonella enterica (subspecies I-VI) and Salmonella bongori were examined using novel primers designed to detect the ompF gene. The target was present in all the 218 Salmonella isolates including all the subspecies of Salm. enterica and Salm. bongori. The ompF gene was absent in 180 non-Salmonella strains tested.

Claims7

Dependent claims

A method of detecting the presence of Salmonella in a sample comprising:

providing a sample suspected of containing Salmonella;
performing an amplification reaction from a nucleic acid preparation derived from the sample using a forward and a reverse primer capable of amplifying a target region of a Salmonella specific gene wherein the gene is ompF;
and
detecting an amplified nucleic acid product generated from the amplification reaction;
wherein the forward primer sequence is substantially similar to SEQ ID NO:
2;
wherein the reverse primer sequence is substantially similar to SEQ ID NO:
3;
wherein the target region of ompF amplified is a 59 base pair region located from nucleotides 18 to 76 of SEQ ID NO:
1.

7 more elements

Dependencies are inferred from the claim text.

Method for detecting Salmonella species by assaying outer membrane Porin F (ompF)

Life of this patent

Method for detecting Salmonella species by assaying outer membrane Porin F (ompF)

Life of this patent

Abstract

Claims7