Method and kit for detecting 5-hydroxymethylcytosine in nucleic acids
AssigneeRiken
InventorsAkimitsu Okamoto, Kaori Sugizaki, Akiko Nakamura, Hiroyuki Yanagisawa, Shuji Ikeda
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Abstract
Provided are a method and a kit for detecting 5-hydroxymethylcytosine in a nucleic acid. The method is a method for detecting 5-hydroxymethylcytosine in a nucleic acid, comprising the steps of: (1) oxidizing 5-hydroxymethylcytosine in a nucleic acid sample by treating the nucleic acid sample with a tungstic acid-based oxidizing agent comprising peroxotungstic acid, tungstic acid, a salt thereof, or a combination thereof with a reoxidizing agent; and (2) determining the position of the oxidized 5-hydroxymethylcytosine in the nucleic acid sample.
Claims8
A method for detecting 5-hydroxymethylcytosine in a nucleic acid, comprising the steps of:
- (1) oxidizing 5-hydroxymethylcytosine possibly present in a nucleic acid sample by treating the nucleic acid sample with a tungstic acid-based oxidizing agent;
- and
- (2) determining the position of the oxidized 5-hydroxymethylcytosine in the nucleic acid sample.
A kit for detecting 5-hydroxymethylcytosine in a nucleic acid, comprising at least one tungstic acid-based oxidizing agent selected from the group consisting of
- (1) peroxotungstic acid, or a salt thereof,
- (2) tungstic acid, or a salt thereof, and
- (3) combinations of peroxotungstic acid, tungstic acid, or a salt thereof with a reoxidizing agent.
Dependencies are inferred from the claim text.