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US 8,895,243 B2Granted
Method of assessing cancerous conditions and reagent for detecting gene product to be used in the method
AssigneeSekisui Medical Co., Ltd.
InventorsMasato Maekawa, Takeshi Uramoto
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Abstract
A reagent for detecting gene product, the reagent comprising a probe or chemical modulation that specifically binds to an alternative splicing junction of a gene product of human PTCH1 gene, the expression of the gene product from gene products of the human PTCH1 gene being varied due to the unusual alternative splicing, for use for measuring the abundance of the gene product contained in a human sample.
Claims6
A method of determining a colon cancer tissue in a human test patient comprising:
- obtaining a first sample from a non-cancerous human subject and a second sample from the test patient,
- measuring a transcription product of the human patched homolog 1 (PTCH1) gene comprising SEQ ID NO:
- 1 in the first and second sample by contacting the samples with a probe or primer that hybridizes to the splice junction in SEQ ID NO:
- 1 or the complement of SEQ ID NO:
- 1, wherein the splice junction is between exon1B and exon 3 of PTCH1, and
- comparing the level of measured transcription product from the first and second samples, wherein if the level of measured transcription product in the second sample is higher than the level of measured transcription product in the first sample, the test patient comprises a colon cancer tissue.
3 more elements
Dependencies are inferred from the claim text.