Enhancing of hepatitis B virus vaccine and its gene
AssigneeFudan University
InventorsNaishuo Zhu, Huaqing Li, Min Yang
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Abstract
This invention relates to a HB vaccine enhancing protein, its gene, gene engineering method for expressing this protein, and the application of this method. The cDNA of this protein, which is screened out from human liver cDNA library, is sequenced and then cloned into prokaryotic or eukaryotic (animal or plant) cell for expression of protein coded by the cDNA (for example, cloning into prokaryotic expression carrier and expression in E. coli) and purification of the protein. The protein obtained, when used with HB vaccine, can significantly increase the effect of the vaccine, the immune power of HBV carrier, and the titer of antibody. The protein can be used as an adjutant to HB vaccine.
Claims2
A method of producing an enhanced Hepatitis B virus (HBV) vaccine, the method comprising:
- combining a first polypeptide having an amino acid sequence of a Hepatitis B surface antigen (HBsAg) with a second polypeptide having an amino acid sequence of a recombinant Hepatitis B surface antigen binding protein (SBP) to produce an enhanced HBV vaccine, wherein
- the second polypeptide consisting of an amino acid sequence of SEQ.ID.NO.2 as an adjuvant, and the enhanced HBV vaccine has greater immunogenicity than the HBsAg itself.
Dependencies are inferred from the claim text.