HCV genotype 4 replicons
AssigneeGilead Sciences, Inc.
InventorsWilliam E. Delaney, IV, Guofeng Cheng, Hongmei Mo, Simin Xu, Betty Peng
Life of this patent
Estimating the term…
Abstract
Replicons of genotype 4 hepatitis C virus (HCV) are provided. These replicons contains adaptive mutations giving rise to the HCV's capability to replicate in vitro. Methods of preparing genotype 4 replicons and methods of using these replicons to screen antiviral agents are also provided.
Claims20
An isolated genotype 4a hepatitis C viral (HCV) RNA construct comprising a 5′NTR, an internal ribosome entry site (IRES), sequence encoding one or more of NS3, NS4A, NS4B, NS5A or NS5B, and a 3′NTR, wherein the RNA construct further comprises a mutation as compared to a wild-type HCV 4a sequence of SEQ ID NO:8, wherein said mutations is T343K, T343R, A200E, or T511R in NS3, or Q34K, Q34R, or E52V in NS4A, or L179P in NS5A, and wherein the mutation positions correspond to the residue positions in SEQ ID NO:9.
Dependencies are inferred from the claim text.