HCV genotype 3 replicons
AssigneeGilead Sciences, Inc.
InventorsWilliam E. Delaney, IV, Guofeng Cheng, Hongmei Mo, Simin Xu, Mei Yu, Amoreena Corsa
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Abstract
Replicons of genotype 3 of hepatitis C virus (HCV) are provided. These replicons contains adaptive mutations giving rise to the HCV's capability to replicate in vitro. Methods of preparing genotype 3 replicons and methods of using these replicons to screen antiviral agents are also provided.
Claims24
An isolated genotype 3a hepatitis C viral (HCV) RNA construct comprising a 5′NTR, an internal ribosome entry site (IRES), sequences encoding one or more of NS3, NS4A, NS4B, NS5A or NS5B, and a 3′NTR, wherein the RNA construct further comprises a mutation as compared to a wild-type HCV 3a sequence of SEQ ID NO:12, wherein said mutation is N607S or P89L, or both, in NS3, and wherein the mutation positions correspond to the residue positions in SEQ ID NO:13.
Dependencies are inferred from the claim text.