Triple cross-linked collagen, method of manufacturing the same, and use thereof

A method for manufacturing a triple cross-linked collagen, comprising the following steps:

• providing a soluble collagen sample;
• mixing the collagen sample with a first cross-linking agent to form a first cross-linked collagen, wherein the first cross-linking agent is one selected from the group consisting of an aldehyde cross-linking agent, and an imine cross-linking agent;
• mixing the first cross-linked collagen with a second cross-linking agent to form a second cross-linked collagen, wherein the second cross-linking agent is one selected from the group consisting of an aldehyde cross-linking agent, and an imine cross-linking agent, and the second cross-linking agent is different from the first cross-linking agent;
• and
• mixing the second cross-linked collagen with a third cross-linking agent to form a triple cross-linked collagen, wherein the third cross-linking agent is an epoxide cross-linking agent;
• wherein the cross-linking reaction for the epoxide cross-linking agent proceeds at pH 3 to pH 5 and 30° C. to 50° C.