Method for expansion of human corneal endothelial cells
OwnerRay Jui-Fang Tsaiinventor
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Abstract
A method for expanding human corneal endothelial cells includes: (a) providing an amniotic membrane with or without amniotic cells, wherein the amniotic membrane has an extracellular matrix; (b) placing onto the amniotic membrane, a sheet of endothelial layer, or a cell suspension including human corneal endothelial stem cells; and (c) culturing the corneal endothelial cells on the amniotic membrane for a duration sufficient for the corneal endothelial stem cells to expand to an appropriate area. The invention also relates to a method for creating a surgical graft for a recipient site of a patient using the method for expanding human corneal endothelial cells, and the surgical graft prepared therefrom.
Claims9
A method for creating a surgical graft, the method comprising:
- (a) culturing human corneal endothelial stem cells on a first substrate to allow expansion of the corneal endothelial stem cells, wherein the first substrate is an amniotic membrane having an extracellular matrix;
- (b) separating the expanded human corneal endothelial stem cells from the amniotic membrane;
- and (c) placing the cells obtained in (b) on a second substrate to obtain the surgical graft;
- wherein the second substrate is a corneal disc or a thin layer of an amniotic membrane.
Dependencies are inferred from the claim text.