Monolignol 4-O-methyltransferases and uses thereof
AssigneeBrookhaven Science Associates, LLC
InventorsChang-Jun Liu, Mohammad-Wadud Bhuiya, Kewei Zhang
Life of this patent
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Abstract
Modified (iso)eugenol 4-O-methyltransferase enzymes having novel capacity for methylation of monolignols and reduction of lignin polymerization in plant cell wall are disclosed. Sequences encoding the modified enzymes are disclosed.
Claims11
A modified (iso)eugenol 4-O-methyltransferase (IEMT) enzyme having three amino acid substitutions selected from the group consisting of enzymes having amino acid substitutions T133L/E165I/F175I and T133L/L139Q/E165F, wherein the modified amino acid residues correspond to those of SEQ ID NO:
- 1.
An isolated nucleic acid molecule encoding a modified (iso)eugenol 4-O-methyltransferase enzyme having three amino acid substitutions selected from the group consisting of sequences encoding amino acid substitutions T133L/E165I/F175I and T133L/L139Q/E165F, wherein the modified amino acid residues correspond to those of SEQ ID NO:
- 1.
A modified (iso)eugenol 4-O-methyltransferase enzyme having four or five amino acid substitutions selected from the group consisting of enzymes having amino acid substitutions T133L/E165I/F175I/F166W, T133L/E165I/F175I/H169F, T133L/E165I/F175I/F166W/H169W, T133L/E165I/F175I/F166W/H169F, T133I/E165I/F175I/Y326F/N327V and T133L/E165I/F175I/F166W/T135N, wherein the modified amino acid residues correspond to those of SEQ ID NO:
- 1.
A modified (iso)eugenol 4-O-methyltransferase (m-IEMT) having an increased activity, compared to wild type (iso)eugenol 4-O-methyltransferase (wt-IEMT), for 4-O-methylation of coniferyl alcohol or sinapyl alcohol or both, said m-IEMT having three, four or five amino acid substitutions selected from the group consisting of T133L/E165I/F175I, T133L/L139Q/E165F, T133L/E165I/F175I/F166W, T133L/E165I/F175H/H169F, T133L/E165I/F175I/F166W/H169W, and T133L/E165I/F175I/F166W/H169F, wherein the modified amino acid residues correspond to those of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.