Diterpene synthases and method for producing diterpenoids
AssigneeThe University of British Columbia
InventorsJoerg Bohlmann, Philipp Zerbe
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Abstract
Provided herein are diterpene synthases (diTPS) and methods for producing diterpenoids. Also provided herein are nucleic acid sequences encoding diTPS, diTPS amino acid sequences, diTPS proteins, vectors, cells, transgenic organisms, uses, compositions, methods, processes, and kits thereof.
Claims22
An isolated nucleic acid molecule, comprising a sequence of nucleotides encoding a cis-abienol synthase (CAS) polypeptide selected from among:
- a) the polypeptide whose sequence is set forth in one of SEQ ID NOS:7, 50 and 55;
- b) an active fragment of the polypeptide of a);
- and
- c) a polypeptide having a sequence of amino acids that has at least 95% sequence identity with a polypeptide of a) or b), wherein:
- the encoded polypeptide or active fragment catalyzes the formation of cis-abienol from geranylgeranyl diphosphate (GGPP);
- and
- the isolated nucleic acid molecule is cDNA.
A host cell, comprising a nucleic acid molecule encoding a cis-abienol synthase (CAS) polypeptide selected from among:
- a) the polypeptide whose sequence is set forth in one of SEQ ID NOS:7, 50 and 55;
- b) an active fragment of the polypeptide of a);
- and
- c) a polypeptide having a sequence of amino acids that has at least 95% sequence identity with a polypeptide of a) or b), wherein:
- the encoded polypeptide or active fragment catalyzes the formation of cis-abienol from geranylgeranyl diphosphate (GGPP);
- and
- the encoded cis-abienol synthase is heterologous to the host cell.
A host cell, comprising a nucleic acid molecule comprising a sequence of nucleotides encoding a cis-abienol synthase (CAS) polypeptide selected from among:
- a) the polypeptide whose sequence is set forth in one of SEQ ID NOS:7, 50 and 55;
- b) an active fragment of the polypeptide of a);
- and
- c) a polypeptide having a sequence of amino acids that has at least 95% sequence identity with a polypeptide of a) or b), wherein:
- the encoded polypeptide or active fragment catalyzes the formation of cis-abienol from geranylgeranyl diphosphate (GGPP);
- and
- the host cell is a yeast or bacterial host cell.
Dependencies are inferred from the claim text.