Biocatalytic production of glycosides
AssigneeUniversiteit Gent
InventorsGiang Hai Tran, Tom Desmet, Wim Soetaert
Life of this patent
Estimating the term…
Abstract
An in vitro method to produce a glycoside is described which includes the steps of contacting the cellodextrin phosphorylase from Clostridium stercorarium with alpha-glucose-1-phosphate or alpha-galactose-1-phosphate and an acceptor, and glycosylating the acceptor. The acceptor may be an alkyl beta-glucoside, an aryl beta-glucoside, a glucolipid, an alkyl beta-sophoroside, an aryl beta-sophoroside or a sophorolipid. Alkylcellobiosides, arylcellobiosides, cellobiolipids, cellotriolipids, glucosophorolipids and cellobiosesophorolipids are produced when alpha-glucose-1-phosphate is used as donor. Corresponding lactosides are produced when alpha-galactose-1-phosphate is used as donor.
Claims15
An in vitro method to produce a glycoside comprising:
- contacting in vitro the cellodextrin phosphorylase from Clostridium stercorarium with alpha-glucose-1-phosphate and an acceptor, and
- glycosylating said acceptor,
- wherein said acceptor is an alkyl beta-glucoside, an aryl beta-glucoside, a glucolipid, an alkyl beta-sophoroside, an aryl beta-sophoroside or a sophorolipid.
An in vitro method to produce a glycoside comprising:
- contacting the cellodextrin phosphorylase from Clostridium stercorarium with alpha galactose-1-phosphate and an acceptor in vitro, and
- glycosylating said acceptor,
- wherein said acceptor is an alkyl beta-glucoside, an aryl beta-glucoside, a glucolipid, an alkyl beta-sophoroside, an aryl beta-sophoroside or a sophorolipid.
Dependencies are inferred from the claim text.