Recombinant codon optimised factor H
AssigneeThe University Court of the University of Edinburgh
InventorsChristoph Schmidt, Paul N. Barlow, Anna Richards
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Abstract
The present invention relates to recombinant factor H and variants and conjugates thereof and methods of their production, as well as uses and methods of treatment involving the materials.
Claims22
A method for making a recombinant mammalian factor H (FH), the method comprising the step of:
- expressing in a bacterial, fungal, insect or plant cell, in culture a codon-optimized nucleic acid sequence encoding the mammalian FH, the nucleic acid sequence comprising a deletion variant sequence of any one of SEQ ID NO:
- 1-13 thereof comprising domains 1-4 and 19-20 and a deletion of domains 8-18, wherein the nucleic acid sequence has been codon optimized to enhance expression in the bacterial, fungal, insect or plant cell and inserted into an expression vector.
A nucleic acid sequence capable of expressing a FH polypeptide wherein the nucleic acid sequence is codon optimized for expression in a bacterial, fungal, insect or plant cell in culture in an amount greater than 1 mg per liter of culture wherein the nucleic acid sequence comprises domains 1-4 and 19-20 and a deletion variant sequence of any one of SEQ ID NOs:
- 1-13 comprising a deletion of domains 8-18.
A recombinantly expressed variant of mammalian FH comprising one or more amino acid residue substitutions designed to modulate one or more biological properties of the FH variant as compared to a native FH, obtained from a nucleic acid sequence capable of expressing a FH polypeptide, wherein the nucleic acid sequence is codon optimized for expression in a bacterial, fungal, insect or plant cell in culture in an amount greater than 1 mg per liter of culture and wherein the nucleic acid sequence comprises a deletion variant sequence of any one of SEQ ID NOs:
- 1-13 comprising domains 1-4 and 19-20, which comprises one or more amino acid substitutions and a deletion of domains 8-18.
A process for making a recombinant mammalian factor H (FH), the process comprising the step of:
- expressing in Pichia pastoris a nucleic acid sequence codon optimized for enhancing expression in P. pastoris encoding the mammalian FH, wherein the nucleic acid sequence comprises a deletion variant sequence of any one of SEQ ID NOs:
- 1-13 comprising domains 1-4 and 19-20 and a deletion of domains 8-18.
Dependencies are inferred from the claim text.