Flavivirus host-range mutations and uses thereof
AssigneeResearch Development Foundation
InventorsDennis T. Brown, Raquel Hernandez
Life of this patent
Estimating the term…
Abstract
Methods and compositions concerning mutant flaviviruses with host-range phenotypes are provided. Nucleotide sequences that encode mutant flavivirus proteins are also provided. In certain aspects, viruses comprising these sequences display reduced replication in mammalian cells. In further aspects of the invention, flavivirus vaccine compositions and methods for vaccination against flavivirus infection are provided.
Claims23
A modified flavivirus envelope protein (E) comprising a E protein N-terminal transmembrane domain (E-T1 domain) having at least one mutation relative to wild type E-T1, such a mutation consisting of a proline substituted for the central glycine, which is designated as position 0 wherein a flavivirus comprising the modified flavivirus E protein has an ability to infect mammalian cells but a reduced ability to replicate therein relative to the wild-type flavivirus.
Dependencies are inferred from the claim text.