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US 8,883,691 B2Granted
Encoded microparticles
AssigneeAffymetrix, Inc.
InventorRandall J. True
Life of this patent
Estimating the term…
Abstract
Microparticles including spatially coded microparticles, systems for imaging and methods of detecting such microparticles as well as using the same in bioassays are provided.
Claims28
A method of detecting a biological analyte comprising:
- (a) delivering a sample suspected of containing a biological analyte to a system, wherein the system comprises a plurality of encoded microparticles, and wherein an encoded microparticle comprises:
- (i) an elongated body comprising a plurality of alternating portions of a more transparent material and a less transparent material, wherein the portions of the less transparent material are adjacent to the portions of more transparent material, and wherein the portions of the more and less transparent materials form a spatial code which is detectable;
- and
- (ii) an outer surface encapsulating the spatial code comprised of glass, silica, silicon dioxide, quartz, silicon nitride, silicon carbide or mixtures thereof;
- wherein the microparticle has a consistent external cross section shape along a length of the microparticle;
- (b) determining the spatial code for individual microparticles;
- and
- (c) determining the presence or absence of a biological analyte by quantifying signals from the plurality of encoded microparticles.
5 more elements
A method of detecting a biological analyte comprising:
- (a) delivering a sample suspected of containing a biological analyte to a system comprising a plurality of encoded microparticles, wherein an encoded microparticle comprises:
- (i) an elongated body comprising a plurality of alternating portions of a first transparent material and a third material, wherein the third material is less transparent than the first transparent material, and wherein the portions of the third material are adjacent to the portions of the first transparent material, and wherein the portions of the first transparent material and the third material form a spatial code which is detectable,
- (ii) an outer surface encapsulating the spatial code comprised of a second transparent material wherein the microparticle has a consistent external cross section shape along a length of the microparticle, and
- (iii) a biological molecule or probe attached to the outer surface, wherein the biological molecule or probe has the capacity to bind to a corresponding biological analyte;
- (b) determining the spatial code for individual microparticles;
- and
- (c) detecting the presence or absence of a biological analyte by determining the presence or absence of a molecular binding event between the biological molecule or probe and the biological analyte.
4 more elements
Dependencies are inferred from the claim text.