Kits comprising linear DNAs for sustained polypeptide expression using synthetic, modified RNAs
AssigneeChildren's Medical Center Corporation
InventorsDerrick Rossi, Luigi Warren
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Abstract
Described herein are synthetic, modified RNAs for changing the phenotype of a cell, such as expressing a polypeptide or altering the developmental potential. Accordingly, provided herein are compositions, methods, and kits comprising synthetic, modified RNAs for changing the phenotype of a cell or cells. These methods, compositions, and kits comprising synthetic, modified RNAs can be used either to express a desired protein in a cell or tissue, or to change the differentiated phenotype of a cell to that of another, desired cell type.
Claims8
A kit for making synthetic mRNAs for the production of induced pluripotent stem (iPS) cells comprising the following components:
- (a) a linear DNA template for the generation of a synthetic modified RNA, the template encoding an OCT4 mRNA of SEQ ID NO:
- 788;
- (b) a linear DNA template for the generation of a synthetic modified RNA, the template encoding a SOX2 mRNA of SEQ ID NO:
- 941 or SEQ ID NO:
- 1501;
- (c) a linear DNA template for the generation of a synthetic modified RNA, the template encoding a KLF4 mRNA of SEQ ID NO:
- 501;
- (d) a linear DNA template for the generation of a synthetic modified RNA, the template encoding a c-MYC mRNA of SEQ ID NO:
- 636;
- and
- (e) a ribonucleoside mixture component comprising 5-methylcytidine and pseudouridine.
Dependencies are inferred from the claim text.