Back to results
US 8,883,466 B2Granted
Bacterial cells exhibiting formate dehydrogenase activity for the manufacture of succinic acid
AssigneeBASF SE
InventorsEdzard Scholten, Stefan Haefner, Hartwig Schröder
Life of this patent
Estimating the term…
Abstract
The present invention relates to a bacterial cell of the genus Pasteurella comprising a heterologous polypeptide having formate dehydrogenase activity. The present invention also relates to a method of manufacturing succinic acid and the use of the bacterial cell for the manufacture of succinic acid.
Claims6
A Pasteurella bacterial cell comprising a heterologous polypeptide having formate dehydrogenase activity, wherein the bacterial cell has increased succinic acid production relative to a corresponding control bacterial cell, the bacterial cell characterized by the 16S rDNA of SEQ ID NO:
- 7 or a 16S rDNA at least 99.9% identical thereto, wherein the heterologous polypeptide having formate dehydrogenase activity is encoded by a polynucleotide comprising a nucleic acid selected from the group consisting of:
- a. a nucleic acid having a nucleotide sequence as shown in SEQ ID NO:
- 5,
- b. a nucleic acid encoding an amino acid sequence as shown in SEQ ID NO:
- 6;
- c. a nucleic acid which is at least 90% identical to the nucleic acid of a) or b);
- and
- d. a nucleic acid encoding an amino acid sequence which is at least 90% identical to the amino acid sequence encoded by the nucleic acid of a) or b).
5 more elements
Dependencies are inferred from the claim text.