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US 8,883,464 B2Granted
Methods for producing 3-hydroxypropionic acid and other products
AssigneesOPX Biotechnologies, Inc., The Regents of the University of Colorado, a body corporate
InventorsMichael D. Lynch, Ryan T. Gill, Tanya E. W. Lipscomb
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Abstract
This invention relates to metabolically engineered microorganism strains, such as bacterial strains, in which there is an increased utilization of malonyl-CoA for production of a chemical product, which includes 3-hydroxypropionic acid.
Claims31
A method for producing an acrylic acid-based consumer product, said method comprising:
- i) combining a carbon source, a microorganism, and cell culture to produce 3-hydroxypropionic acid, wherein:
- a) said cell culture comprises an inhibitor of fatty acid synthase or said microorganism is genetically modified to comprise a deletion of an enzyme, or a substitution of an enzyme with a mutated version of said enzyme, wherein said enzyme selected from the group consisting of:
- malonyl-CoA-ACP transacylase, beta-ketoacyl-ACP synthase I, beta-ketoacyl-acyl carrier protein synthase II, and enoyl-ACP reductase, thereby providing for reduced conversion of malonyl-CoA to fatty acids;
- and
- b) wherein said microorganism is genetically modified for increased enzymatic activity in the microorganism's malonyl-CoA reductase (mcr) pathway by introduction of a heterologous nucleic acid sequence coding for a polypeptide having mono- or bi-functional malonyl-CoA reductase activity;
- ii) converting said 3-hydroxypropionic acid to acrylic acid;
- and
- iii) processing said acrylic acid into a consumer product.
5 more elements
Dependencies are inferred from the claim text.