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AssigneeKorea Research Institute of Bioscience and Biotechnology
InventorsDong-Shan An, Song-Gun Kim, Sung-Taik Lee, Wan-Taek Im, Chang-Hao Cui
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The present invention relates to Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase and use thereof. The polypeptide has an activity of converting PPD (protopanaxadiol)-type saponins into in vivo absorbable and highly active deglycosylated saponins, by selective hydrolysis of a bond at a particular position of ginsenoside. The present invention also relates an amino acid sequence constituting the polypeptide, a nucleic acid sequence encoding the protein, a recombinant vector comprising the nucleic acid sequence, and a transformant transformed with the vector. The invention further provides a method for preparing Rhodanobacter ginsenosidimutans KCTC22231T-derived ginsenoside glycosidase by culturing the transformant, a method for converting PPD (protopanaxadiol)-type major ginsenoside into a rare ginsenoside that is absorbable in vivo using the protein, and a composition for converting PPD-type saponins into in vivo absorbable saponins, having the protein as an active ingredient.
A recombinant vector comprising a nucleotide sequence that encodes a polypeptide having an amino acid sequence shown in SEQ ID NO. 2 and is operably linked to regulatory elements that direct the production of said polypeptide in an expression host.
Dependencies are inferred from the claim text.