Lens-free wide-field super-resolution imaging device

A lens-free system for imaging objects within a sample comprising:

• stationary image sensor and a sample disposed adjacent to the image sensor at a distance z2;
• one or more illumination sources comprising partially coherent or incoherent light in the visible spectrum coupled to an array of optical waveguides, wherein each optical waveguide of the array terminates at a different spatial location in three dimensional space and at a distance z1 from the sample, wherein z2<<z1 and wherein the image sensor acquires a lens-free in-line low resolution hologram image of the objects within the sample from light emitted at the different spatial locations;
• and
• at least one processor configured to reconstruct a single high resolution image of the objects within the sample based on the acquired lens-free in-line low resolution hologram images of the objects within the sample obtained from light emitted at the plurality of different spatial locations.

A lens-free system for imaging a sample comprising:

• a stationary image sensor and a sample disposed adjacent to the image sensor at a distance z2;
• an illumination source comprising an array of partially coherent or incoherent light sources in the visible spectrum coupled to respective optical waveguides terminating at physically separate locations that are separated from each other in the range of about 0.001 mm to about 500 mm and located at a distance z1 from the sample, wherein z2<<z1 and wherein the image sensor acquires a lens-free in-line low resolution hologram image of the objects within the sample at each of the plurality of waveguides;
• and
• at least one processor configured to reconstruct a single high resolution image of the objects within the sample based on the acquired lens-free in-line low resolution hologram image of the objects within the sample obtained with light emitted from the plurality of waveguides.

A method of lens-free imaging of objects within a sample comprising:

• illuminating a sample with an illumination source emitting partially coherent or incoherent light in the visible spectrum at a first position through at least one of an aperture or an optical waveguide;
• obtaining a lens-free in-line low resolution hologram image of the objects within the sample from an image sensor at the first position;
• illuminating the sample with the illumination source at a plurality of additional positions;
• obtaining a plurality of additional lens-free in-line low resolution hologram images of the objects within the sample at each of the plurality of additional positions;
• and
• recovering a single high resolution image of the objects within the sample based at least in part on the plurality of lens-free in-line low resolution hologram images.

A lens-free system for imaging a sample comprising:

• a stationary image sensor and a sample disposed adjacent to the image sensor at a distance z2;
• an illumination source comprising an array of partially coherent or incoherent light sources in the visible spectrum configured to direct light through a plurality of apertures located at physically separate locations that are separated from each other in the range of about 0.001 min to about 500 mm and located at a distance z1 from the sample, wherein z2<<z1 and wherein the image sensor acquires a lens-free in-line low resolution hologram image of the objects within the sample with light emitted through each of the plurality of apertures;
• and
• at least one processor configured to reconstruct a single high resolution image of the objects within the sample based on the acquired lens-free in-line low resolution hologram images of the objects within the sample obtained from light emitted through each of the physical apertures.