Transgenic algae engineered for higher performance
AssigneesLos Alamos National Security, LLC, University of Maine System Board of Trustees
InventorsPat J. Unkefer, Penelope S. Anderson, Thomas J. Knight
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to transgenic algae having increased growth characteristics, and methods of increasing growth characteristics of algae. In particular, the disclosure relates to transgenic algae comprising a glutamine phenylpyruvate transaminase transgene and to transgenic algae comprising a glutamine phenylpyruvate transaminase transgene and a glutamine synthetase.
Claims10
A transgenic alga comprising a plant-derived or algal-derived glutamine phenylpyruvate transaminase (GPT) transgene and a glutamine synthetase (GS) transgene, wherein each of said GPT transgene and said GS transgene is operably linked to a promoter, wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of:
- SEQ ID NO:
- 2, SEQ ID NO:
- 9, SEQ ID NO:
- 15, SEQ ID NO:
- 19, SEQ ID NO:
- 21, SEQ ID NO 24, SEQ ID NO:
- 30, SEQ ID NO:31, SEQ ID NO:
- 32, SEQ ID NO:
- 33, SEQ ID NO:
- 34, SEQ ID NO:
- 36, SEQ ID NO:
- 44, SEQ ID NO:
- 47, and 48.
A method for increasing growth characteristics of an alga relative to an wild type or progenitor alga of the same species, comprising:
- (a) introducing a plant-derived or algal-derived GPT transgene into the alga wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of:
- SEQ ID NO:
- 2, SEQ ID NO:
- 9, SEQ ID NO:
- 15, SEQ ID NO:
- 19, SEQ ID NO:
- 21, SEQ ID NO 24, SEQ ID NO:
- 30, SEQ ID NO:31, SEQ ID NO:
- 32, SEQ ID NO:
- 33, SEQ ID NO:
- 34, SEQ ID NO:
- 36, SEQ ID NO:
- 44, SEQ ID NO:
- 47, and 48;
- (b) introducing a GS trans gene into the alga or a progeny of the alga;
- (c) expressing the GPT transgene and the GS transgene in the alga or the progeny of the alga;
- and,
- (d) selecting an alga having an increased growth characteristic relative to an alga of the same species that does not comprise the GPT transgene or the GS transgene.
Dependencies are inferred from the claim text.