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US 8,859,846 B2Granted
Doubling of chromosomes in haploid embryos
AssigneesE. I. Du Pont de Nemours and Company, Pioneer Hi-Bred International, Inc.
InventorsJoanne E. Barton, Sheila E. Maddock, Xinli E. Wu, Zuo-Yu Zhao, Mark E. Williams, Tanveer Hussain, William J. Gordon-Kamm
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Abstract
Methods for producing doubled haploid corn (Zea mays) plants, seeds, and plant cells are provided.
Claims10
A method of obtaining a doubled haploid maize plant, said method comprising:
- (a) pollinating silks of a maize ear with a maize inducer line to produce at least one diploid maize embryo and at least one haploid maize embryo;
- (b) isolating said haploid maize embryo between 4-21 days after step (a), wherein said at least one haploid maize embryo is distinguished from the diploid maize embryos via expression of a marker;
- (c) contacting said haploid maize embryo with a chromosome doubling agent to produce at least one doubled haploid maize embryo cell;
- (d) culturing said doubled haploid maize embryo cell on a non-callus promoting medium;
- and
- (e) generating a doubled haploid maize plant from said doubled haploid maize embryo cell.
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Dependencies are inferred from the claim text.