Self-assembling cell aggregates and methods of making engineered tissue using the same
AssigneesThe Curators of the University of Missouri, Medical University of South Carolina, MUSC Foundation for Research Development
InventorsGabor Forgacs, Karoly Jakab, Adrian Neagu, Vladimir Mironov
Life of this patent
Estimating the term…
Abstract
A method of making engineered tissue from a plurality of cell aggregates is disclosed. A cell suspension is centrifuged. The resulting pellet is extruded through an orifice, and the extruded pellet is cut into pieces to produce cell aggregates. A plurality of the cell aggregates are printed in a pattern, and allowed to fuse to form a desired three-dimensional engineered tissue structure. Modeling methods predict the structural evolution of fusing cell aggregates for combinations of cell type to enable selection of organ printing process parameters for use in producing an engineered tissue having a desired three-dimensional structure.
Claims23
A method of producing a three-dimensional engineered biological tissue, the method comprising:
- arranging a plurality of cell aggregates according to a pattern such that each of the cell aggregates contacts at least one other cell aggregate, wherein each cell aggregate comprises a plurality of living cells, and
- allowing at least one of the cell aggregates to fuse with at least one other cell aggregate to produce a three-dimensional engineered biological tissue.
Dependencies are inferred from the claim text.