Methods for detecting Lp-PLA2 activity and inhibition of Lp-PLA2 activity
AssigneeGlaxo Group Limited
InventorsYaping Shou, Yin-Fai Siu, George T. Walker
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Abstract
This invention relates to methods for determining the activity of Lp-PLA2 in at least one sample from an animal. The invention also relates to methods for determining the inhibition of Lp-PLA2 activity in samples from animals that are administered an Lp-PLA2 inhibitor.
Claims38
A method for determining inhibition of Lp-PLA2 enzyme activity in at least one sample comprising the steps of:
- (a) preparing a solution comprising a substrate for lipoprotein-associated phospholipase A2 (“Lp-PLA2”) comprising a colorimetric or fluorometric detectable moiety;
- (b) contacting at least one said sample with the solution of step (a);
- and
- (c) detecting Lp-PLA2 activity;
- wherein the sample is from an animal that has been administered an Lp-PLA2 inhibitor and wherein the sample is diluted about 3 to about 9 fold by contact with the solution of step (a).
A method for determining Lp-PLA2 enzyme activity in a sample obtained from an animal that has been administered an Lp-PLA2 inhibitor comprising the steps of:
- a) contacting said sample with a solution comprising a substrate for Lp-PLA2 comprising a colorimetric or fluorometric detectable moiety;
- and
- b) detecting Lp-PLA2 activity;
- wherein said sample is diluted about 3 to 9 fold by contact with the solution comprising a substrate.
A method for determining inhibition of Lp-PLA2 enzyme activity in at least one sample comprising the steps of:
- (a) preparing a solution comprising a substrate for lipoprotein-associated phospholipase A2 (“Lp-PLA2”);
- (b) contacting at least one said sample with the solution of step (a);
- and
- (c) fluorometrically or colorimetrically detecting Lp-PLA2 activity;
- wherein the sample is from an animal that has been administered an Lp-PLA2 inhibitor and wherein the sample is diluted about 3 to about 9 fold by contact with the solution of step (a).
A method for determining Lp-PLA2 enzyme activity in a sample obtained from an animal that has been administered an Lp-PLA2 inhibitor comprising the steps of:
- a) contacting said sample with a solution comprising a substrate for Lp-PLA2;
- and
- b) fluorometrically or colorimetrically detecting Lp-PLA2 activity;
- wherein said sample is diluted about 3 to 9 fold by contact with the solution comprising a substrate.
Dependencies are inferred from the claim text.