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US 8,841,071 B2Granted
Sample multiplexing
AssigneeRaindance Technologies, Inc.
InventorDarren Link
Life of this patent
Estimating the term…
Abstract
The invention generally relates to methods for sample multiplexing. In certain embodiments, methods of the invention obtaining a plurality of different reactant molecules, attaching a unique identifier to the reactant molecules, and forming a droplet including the reactant molecules.
Claims21
A method for preparing a plurality of droplets, the method comprising:
- obtaining a plurality of reactant molecules from different samples;
- attaching a unique identifier to said reactant molecules from each sample so that the reactant molecules from a particular sample can be differentiated from the reactant molecules of other samples;
- and
- forming a plurality of droplets, each droplet comprising reactant molecules from more than one of the different samples and at least one primer pair, wherein a first primer of the primer pair is complementary to a sequence of one of the reactant molecules and a second primer of the primer pair is complementary to a portion of the unique identifier.
1 more element
A method for forming a droplet that allows for the differentiation of nucleic acids from different samples, the method comprising:
- forming a droplet comprising a primer pair and nucleic acid from different samples, wherein an oligonucleotide sequence comprising a unique sequence portion is attached to the nucleic acids from each sample, so that the nucleic acids from a particular sample can be differentiated from nucleic acids of other samples and a first primer of the primer pair is complementary to a sequence of one of the reactant molecules and a second primer of the primer pair is complementary to a portion of the oligonucleotide sequence.
Dependencies are inferred from the claim text.