Method for transfer of gene into fat cell or progenitor fat cell
AssigneeTakara Bio Inc.
InventorsTakahiro Marui, Tatsuji Enoki, Hiroaki Sagawa, Ikunoshin Kato
Life of this patent
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Abstract
A method for transferring a gene into a fat cell or progenitor fat cell comprising the step of infecting the fat cell or progenitor cell with a retrovirus vector having a foreign gene in the presence of a substance having both of a retrovirus-binding site and a target cell-binding site in the molecule or a mixture of a substance having a retrovirus-binding site and a substance having a target cell-binding site, the target cell-binding site having a region that can bind to VLA-5 and/or a region that can bind to VLA-4.
Claims4
A method for increasing the efficiency transferring a gene into an adipocyte or a preadipocyte, the method comprising infecting an adipocyte or a preadipocyte with the proviso that the preadipocyte is a cell that is capable of directly differentiating into an adipocyte and is not a mesenchymal stem cell or stromal cell which retains abilities to differentiate into various cell types with a retrovirus vector having a foreign gene in the presence of a polypeptide having the amino acid sequence of:
- (a) SEQ ID NO:1 and SEQ ID NO:2;
- (b) SEQ ID NO:1 and SEQ ID NO:3;
- or
- (c) SEQ ID NO:1 and SEQ ID NO:2 and SEQ ID NO:3.
In a method for transferring a gene into an adipocyte or a preadipocyte, the improvement comprising infecting an adipocyte or a preadipocyte with the proviso that the preadipocyte is a cell that is capable of directly differentiating into an adipocyte and is not a mesenchymal stem cell or stromal cell which retains abilities to differentiate into various cell types with a retrovirus vector having a foreign gene in the presence of a polypeptide having the amino acid sequence of:
- (a) SEQ ID NO:1 and SEQ ID NO:2;
- (b) SEQ ID NO:1 and SEQ ID NO:3;
- or
- (c) SEQ ID NO:1 and SEQ ID NO:2 and SEQ ID NO:3.
Dependencies are inferred from the claim text.