Cellular production of glucaric acid through recombinant expression of uronate dehydrogenase and myo-inositol oxygenase
AssigneeMassachusetts Institute of Technology
InventorsTae Seok Moon, Sang-Hwal Yoon, Kristala Lanett Jones Prather
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Abstract
The invention relates to the production of glucuronic and glucaric acid in cells through recombinant expression of myo-inositol 1-phosphate synthase, myo-inositol oxygenase and uronate dehydrogenase. Cloning and characterization of the gene encoding uronate dehydrogenase is also disclosed.
Claims27
An isolated cell that recombinantly expresses a protein comprising the polypeptide set forth by SEQ ID NO:
- 2, 24, or 26, or a polypeptide that has least 95% identity to SEQ ID NO:
- 2, 24, or 26, wherein the polypeptide has uronate dehydrogenase activity, and wherein the cell produces glucaric acid.
An isolated microorganism that recombinantly expresses a protein comprising the polypeptide set forth by SEQ ID NO:
- 2, 24, or 26, or a polypeptide that has least 95% identity to SEQ ID NO:
- 2, 24, or 26, wherein the polypeptide has uronate dehydrogenase activity, and wherein the microorganism produces glucaric acid.
A recombinant expression vector comprising a transcription regulatory element linked to an isolated nucleic acid molecule selected from the group consisting of:
- (a) an isolated nucleic acid molecule comprising SEQ ID NO:
- 1, SEQ ID NO:23, or SEQ ID NO:25;
- (b) an isolated nucleic acid molecule encoding an amino acid sequence comprising the sequence of SEQ ID NO:2, SEQ ID NO:24 or SEQ ID NO:26;
- (c) an isolated nucleic acid molecule that is a reverse complement of the full-length sequence of (a) or (b);
- and
- (d) an isolated nucleic acid molecule that has at least 95% nucleotide identity to any one of (a)-(c) wherein the nucleic acid molecule encodes a uronate dehydrogenase.
Dependencies are inferred from the claim text.