Thrombolytic enzyme and a process for its preparation
AssigneesNational Research Development Corporation, India and Malladi Drugs Pharmaceuticals Ltd.
InventorsPerurmadom Ramaiyer Mahadevan, Subrahamanyam Chivukula Sekar, Sundaramurthy Suresh Babu
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Abstract
The invention relates to a thrombolytic enzyme referred to as Thrombinase having a molecular weight of 31,000 to 32,000. Such a thrombolytic enzyme can be used for dissolving blood clots. The process comprises culturing a filtrate of Bacillus sphaericus sero type H5a 5b, removing the cell, subjecting the cell supernatant to filtration, salting out the retentate, subjecting the precipitate to dialysis, reprecipitating the precipitate and then reconstituting in buffer and finally decolorizing, purifying and dialyzing.
Claims12
A biologically purified and isolated thrombinase having a molecular weight in the range of 31,000 to 32,000 Daltons useful in dissolving blood clots for medical conditions.
A method of treating cerebral thrombosis, myocardial infraction and deep vein thrombosis comprising administering thrombinase having a molecular weight in the range of 31,000 to 32,000 to a patient in need thereof and thereby dissolving in vivo a blood clot of said patient.
Dependencies are inferred from the claim text.