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US 8,829,166 B2Granted
Rapid isolation of osteoinductive protein mixtures from mammalian bone tissue
AssigneeZimmer Orthobiologics, Inc.
InventorsKevin Thorne, Rama Akella
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Abstract
A method for purifying bone-derived osteoinductive proteins including a demineralization process, a protein extraction process, a high molecular weight ultrafiltration process, a low molecular weight ultrafiltration process, and a recover process. The high and low ultrafiltration processes preferably select proteins having a nominal molecular weight between approximately 8 kilodaltons and approximately 100 kilodaltons. Processes of the present invention may be used to recover osteoinductive proteins from bone demineralization waste streams.
Claims29
A method of producing an osteoinductive composition, comprising:
- contacting bone tissue with a demineralization medium consisting essentially of an acidic solution and optionally a bone mineral solubility enhancer, a defoaming agent or both to provide demineralized bone tissue and a mineralized supernatant;
- separating the mineralized supernatant from the demineralized bone tissue;
- recovering osteoinductive proteins from the mineralized supernatant;
- and
- combining the osteoinductive proteins with a carrier.
2 more elements
Dependencies are inferred from the claim text.