Method of myelinating isolated motoneurons

A method of making myelinated motoneurons in vitro, the method comprising:

• co-culturing isolated motoneurons and Schwann cells in a serum-free medium of Table 1 on a surface having thereon a covalently attached monolayer of N-1[3(trimethoxysilyl)propyl]diethylenetriamine;
• and
• passaging the co-culture as necessary into fresh medium of Table 1 supplemented with L-ascorbic acid until the motoneurons are myelinated and Nodes of Ranvier are formed thereon.

An isolated motoneuron myelinated in vitro by a method comprising co-culturing isolated motoneurons and Schwann cells in a serum-free medium of Table 1 on a surface having thereon a covalently attached monolayer of N-1[3(trimethoxysilyl)propyl]diethylenetriamine;

• and passaging the co-culture as necessary into fresh serum-free medium of Table 1 supplemented with L-ascorbic acid until the motoneurons are myelinated and Nodes of Ranvier are formed thereon.

A culture of motoneurons myelinated in vitro by a method comprising co-culturing isolated motoneurons and Schwann cells in a serum-free medium of Table 1 on a surface having thereon a covalently attached monolayer of N-1[3(trimethoxysilyl)propyl]diethylenetriamine;

• and passaging the co-culture as necessary into fresh serum-free medium of Table 1 supplemented with L-ascorbic acid until the motoneurons are myelinated and Nodes of Ranvier are formed thereon.

A mixed culture of isolated Schwann cells and motoneurons in a serum-free medium of Table 1, wherein the motoneurons are myelinated.

An isolated dysfunctional motoneuron remyelinated by a method-comprising co-culturing isolated motoneurons and Schwann cells in a serum-free medium of Table 1 on a surface having thereon a covalently attached monolayer of N-1[3(trimethoxysilyl)propyl]diethylenetriamine;

• and passaging the co-culture as necessary into fresh serum-free medium of Table 1 supplemented with L-ascorbic acid until the motoneurons are myelinated and Nodes of Ranvier are formed thereon.