Method for producing isopropanol and recombinant yeast capable of producing isopropanol
AssigneeToyora Jidosha Kabushiki Kaisha
InventorsMasayoshi Muramatsu, Satoshi Yoneda
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Abstract
Isopropanol is produced with good productivity via fermentation processes. Specifically, isopropanol is produced at a high level in a medium by culturing recombinant yeast into which an acetoacetyl CoA synthase gene and a group of genes (isopropanol synthesis-related gene group) encoding a group of enzymes for synthesis of isopropanol from acetoacetyl CoA have been introduced.
Claims3
A method for producing isopropanol, comprising culturing recombinant yeast into which a heterologous acetoacetyl CoA synthase gene encoding an enzyme that catalyzes a reaction for conversion of acetyl CoA and malonyl CoA to acetoacetyl CoA and an isopropanol-biosynthesis-related gene group associated with a metabolic pathway for synthesis of isopropanol from acetoacetyl CoA are introduced, wherein the acetoacetyl CoA synthase gene is a gene (ORFn gene) obtained from a microorganism of the genus Streptomyces and the isopropanol-biosynthesis-related gene group comprises an isopropanol dehydrogenase gene (pdh gene) from a Clostridium-beijerinckii, thereby obtaining a recombinant yeast expressing the ORFn gene and the pdh gene, and then obtaining isopropanol from the culture product of the recombinant yeast.
Dependencies are inferred from the claim text.