US 8,822,662 B2 · Desaturases and process for the production of polyunsaturated fatty acids in transgenic organisms

Abstract

The present invention relates to polynucleotides from Cochliobolus heterostrophus C5, Cyanothece sp. CCY0110, Mycocentrospora acerina and Hyaloperonospora parasitica, which code for desaturases and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotides according to the invention, and to the polypeptides encoded by the polynucleotides. The invention furthermore relates to antibodies against the polypeptides according to the invention. Finally, the invention also relates to production processes for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.

Claims19

Dependent claims

A polynucleotide comprising a nucleic acid sequence operably linked to a heterologous expression control sequence, wherein said nucleic acid sequence is selected from the group consisting of:

(a) the nucleic acid sequence of SEQ ID NO:
1 or 2;
(b) a nucleic acid sequence which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
3;
and
(c) a nucleic acid sequence which encodes a polypeptide having Δ15-desaturase activity and comprising an amino acid sequence having at least 70% sequence identity to the amino acid sequence of SEQ ID NO:
3,
wherein said polypeptide comprises the desaturase motif 2 of SEQ ID NO:
47, and wherein position 16 of SEQ ID NO:
47 is histidine.

7 more elements

Dependencies are inferred from the claim text.

Facts

Application

13/139,014Filed 8 Dec 2009

Priority

EP 0817152012 Dec 2008

Published

US 2011/0277043 A110 Nov 2011

Granted

2 Sep 2014

Term ends

Not estimatedThe source records don’t support an estimate.

Classification14 codes

C12P 7/6409
Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group;…:…
C12N 9/0071
Oxidoreductases: acting on paired donors with incorporation of molecular oxygen
C12N 9/0083
Acting on paired donors with incorporation of molecular oxygen: miscellaneous
C12N 15/8247
Involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine: involving modified lipid metabolism, e.g. seed…

Desaturases and process for the production of polyunsaturated fatty acids in transgenic organisms

Life of this patent

Desaturases and process for the production of polyunsaturated fatty acids in transgenic organisms

Life of this patent

Abstract

Claims19