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US 8,818,734 B1Granted
Peptide ligands for sperm DNA fragmentation assay
OwnerDagan Wells, Jacques Cohen +1inventors
InventorsDagan Wells, Jacques Cohen, George Pieczenik
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Abstract
This invention comprises peptides, methods and a kit for identifying DNA fragmentation in non-viable sperm. Specific peptide sequences are claimed that bind to ssDNA and fragmented DNA.
Claims6
A ssDNA binding peptide comprising the sequence of SEQ ID NO:2.
A viable sperm determining kit comprising Annexin V and a ssDNA and fragmented DNA binding peptide comprising the peptide of SEQ ID NO:2.
A method of detecting viable sperm comprising the steps of:
- a) staining sperm with chromophore labeled Annexin V and,
- b) staining sperm with a different chromophore labeled peptide of SEQ ID 2 and,
- c) identifying those sperm not stained which are viable.
A method of detecting viable sperm comprising the steps of:
- a) staining the sperm with a dye selected from Hoecht, DAPI, and Sytox Green to determine viability, and
- b) staining additionally with the peptide of SEQ ID NO:2;
- and,
- c) identifying those viable sperm which do not stain with the peptide of SEQ ID NO:2.
1 more element
Dependencies are inferred from the claim text.