Hybrid tissue scaffold for tissue engineering
AssigneeSouthwest Research Institute
InventorsJeffrey Nelson Harris, Jian Ling, Xingguo Cheng
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Abstract
A hybrid tissue scaffold is provided which comprises a porous primary scaffold having a plurality of pores and a porous secondary scaffold having a plurality of pores, wherein the secondary scaffold resides in the pores of the primary scaffold to provide a hybrid scaffold. The pores of the porous primary scaffold may have a pore size in a range of 0.50 mm to 5.0 mm, and the pores of the porous secondary scaffold may have a pore size in a range of 50 μm to 600 μm. The primary scaffold may provide 5% to 30% of a volume of the hybrid scaffold.
Claims14
A method of forming a hybrid tissue scaffold comprising:
- forming a solution of biodegradable polymer in the presence of an inorganic salt wherein the inorganic salt is water soluble and has a particulate size in a range of 0.50 to 5.0 mm;
- cooling said solution to precipitate said biodegradable polymer followed by treatment with water to dissolve said inorganic salt wherein the biodegradable polymer is recovered as a porous primary scaffold having a pore size in a range of 1 mm to 4 mm;
- introducing into said primary scaffold a biomaterial comprising a hydrogel including living cells and forming a secondary scaffold from said biomaterial including living cells, wherein the secondary scaffold resides in the pores of the primary scaffold to provide a hybrid scaffold;
- wherein said biomaterial provides an environment to culture the living cells and said biomaterial has a pore size of 50 μm to 600 μm;
- and
- wherein said primary scaffold is present at 5.0% to 30.0% by volume of said hybrid scaffold and said hybrid scaffold has a Young's modulus of 0.1 MPA to 100 GPa.
A method of forming a hybrid tissue scaffold comprising:
- mixing biodegradable polymer particulate size in a range of 0.50 mm to 5.0 mm with an inorganic salt having a particulate size in a range of 0.5 mm to 5.0 mm;
- sintering or treating said biodegradable polymer with solvent to form a continuous phase of the biodegradable polymer around said salt;
- dissolving said inorganic salt wherein said biodegradable polymer is recovered as a porous primary scaffold having a pore size in a range of 1 mm to 4 mm;
- introducing into said primary scaffold a biomaterial comprising a hydrogel including living cells and forming a secondary scaffold from said biomaterial including living cells, wherein the secondary scaffold resides in the pores of the primary scaffold to provide a hybrid scaffold;
- wherein said biomaterial provides an environment to culture the living cells and said biomaterial has a pore size of 50 μm to 600 μm;
- and
- wherein said primary scaffold is present at 5.0% to 30.0% by volume of said hybrid scaffold and said hybrid scaffold has a Young's modulus of 0.1 MPA to 100 GPa.
Dependencies are inferred from the claim text.