Device for rapid determination of disease-associated thiol compounds
AssigneeAlt Bioscience, LLC
InventorsAnthony Burgess-Cassler, Boyd E. Haley
Life of this patent
Estimating the term…
Abstract
Disclosed is a single-use disposable article for determining the concentration of thiol compounds in a sample of fluid, a single-use disposable test strip for determining the concentration of thiol compounds in a sample of a fluid from a mammalian subject, a single-use disposable test strip for determining the risk that a mammalian subject is suffering from a particular malady, and a description of how to make and use the article and test strips.
Claims31
A single-use disposable article for determining the concentration of thiol compounds in a sample of fluid, the article comprising:
- (a) a matrix comprising at least one absorbent material comprising a polyamide material, the matrix being capable of absorbing the sample;
- (b) an amount of a detection reagent, the amount of the detection reagent being capable of providing, during a period of time after at least a portion of the amount of the detection reagent has been contacted with at least a portion of the sample, a signal indicating the concentration of thiol compounds in the sample;
- and
- (c) a comparator region,
- wherein the detection reagent comprises 10 mg/L to 150 mg/L 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), 20 mM to 150 mM imidazole or phosphate buffer, about 0.1 mM manganese chloride, 0.1% to 5.0% by volume glycerol, and 2% to 10% by weight xylitol.
A single-use disposable test strip for determining the concentration of thiol compounds in a sample of a fluid from a mammalian subject, the test strip comprising:
- (a) a matrix comprising at least one absorbent fiber material comprising a polyamide material, the matrix being capable of absorbing the sample;
- (b) an amount of a detection reagent comprising 10 mg/L to 150 mg/L 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), 20 mM to 150 mM imidazole or phosphate buffer, about 0.1 mM manganese chloride, 0.1% to 5.0% by volume glycerol, and 2% to 10% by weight xylitol, the amount of the detection reagent being capable of providing, during a period of time after at least a portion of the amount of the detection reagent has been contacted with at least a portion of the sample, a signal indicating the concentration of thiol compounds in the sample;
- and
- (c) a comparator region.
A single-use disposable test strip for determining the risk that a mammalian subject is suffering from a particular malady, the test strip comprising:
- (a) a matrix comprising at least one absorbent material comprising a polyamide material, the matrix being capable of absorbing a sample from the subject;
- (b) an amount of a detection reagent comprising 10 mg/L to 150 mg/L 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), 20 mM to 150 mM imidazole or phosphate buffer, about 0.1 mM manganese chloride, 0.1% to 5.0% by volume glycerol, and 2% to 10% by weight xylitol, the amount of the detection reagent being capable of providing, during a period of time after at least a portion of the amount of the detection reagent has been contacted with at least a portion of the sample, a signal indicating the risk that the subject is suffering from the particular malady, and wherein
- the particular malady is at least one of:
- periodontitis, gingivitis, halitosis, cardiovascular disease, type II diabetes, or preterm delivery;
- and
- (c) a comparator region.
A single-use disposable article for determining the concentration of thiol compounds in a sample of fluid, the article comprising:
- (a) a matrix comprising at least one absorbent material comprising a polyamide material, the matrix being capable of absorbing the sample;
- (b) an amount of a detection reagent, the amount of the detection reagent being capable of providing, during a period of time after at least a portion of the amount of the detection reagent has been contacted with at least a portion of the sample, a signal indicating the concentration of thiol compounds in the sample;
- and
- (c) a comparator region,
- further comprising a flavoring or scent.
A single-use disposable article for determining the concentration of thiol compounds in a sample of fluid, the article comprising:
- (a) a matrix comprising at least one absorbent material comprising a polyamide material, the matrix being capable of absorbing the sample;
- (b) an amount of a detection reagent, the amount of the detection reagent being capable of providing, during a period of time after at least a portion of the amount of the detection reagent has been contacted with at least a portion of the sample, a signal indicating the concentration of thiol compounds in the sample;
- and
- (c) a comparator region,
- wherein the dried detection reagent is stable for more than one year at room temperature.
A single-use disposable article for determining the concentration of thiol compounds in a sample of fluid, the article comprising:
- (a) a matrix comprising at least one absorbent material, the matrix being capable of absorbing the sample;
- (b) an amount of a detection reagent comprising 10 mg/L to 150 mg/L 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), 20 mM to 150 mM imidazole or phosphate buffer, about 0.1 mM manganese chloride, 0.1% to 5.0% by volume glycerol, and 2% to 10% by weight xylitol, the amount of the detection reagent being capable of providing, during a period of time after at least a portion of the amount of the detection reagent has been contacted with at least a portion of the sample, a signal indicating the concentration of thiol compounds in the sample;
- and
- (c) a comparator region.
Dependencies are inferred from the claim text.