Genetically modified organisms for increased microbial production of 3-hydroxypropionic acid involving an oxaloacetate alpha-decarboxylase
AssigneeOPX Biotechnologies, Inc.
InventorsMichael D Lynch, Christopher P. Mercogliano
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Abstract
Microorganism compositions are described that comprise combinations of genetic modifications that include a genetic modification to increase oxaloacetate alpha-decarboxylase enzymatic activity. By such genetic modification a 3-hydroxypropionic acid (“3-HP”) production pathway is provided or improved. In various embodiments, comprising other genetic modifications, including selected gene disruptions, 3-HP production is greater than in a control microorganism lacking such combinations of genetic modifications.
Claims26
A genetically modified microorganism comprising:
- i) a heterologous nucleic acid sequence encoding a 3 hydroxypropionate dehydrogenase;
- ii) a heterologous nucleic acid sequence encoding a cyanase or a carbonic anhydrase;
- and
- iii) a heterologous nucleic acid sequence encoding an NADPH-dependent transhydrogenase or an acetyl-CoA carboxylase.
A genetically modified microorganism comprising:
- i) a heterologous nucleic acid sequence encoding an enzyme selected from the group consisting of:
- a native or mutated form of a mmsB protein, and a native or mutated form of a ydfG protein,
- ii) a genetic modification to decrease or eliminate an enzymatic activity selected from the group consisting of:
- lactate dehydrogenase, phosphate acetyltransferase, and pyruvate-formate lyase;
- iii) a heterologous nucleic acid sequence encoding an acetyl-CoA carboxylase;
- and
- iv) a heterologous nucleic acid sequence encoding a cyanase.
A genetically modified microorganism comprising:
- i) a heterologous nucleic acid sequence encoding a 3-hydroxypropionate dehydrogenase;
- and
- ii) a heterologous nucleic acid sequence encoding a cyanase or a carbonic anhydrase.
Dependencies are inferred from the claim text.