Detection method for intermolecular interaction and detection device thereof

A detection method for an intermolecular interaction comprising:

• a step of shining light on a ligand;
• a step of measuring, by a spectrometer, light reflected by the ligand,
• a step of calculating, by a control device from the reflected light, a reflectance at a fixed wavelength interval to obtain a reflectance spectrum;
• a step of filtering, by the control device, the reflectance spectrum;
• a step of selecting, by the control device from the reflectance spectrum after being filtered, a first wavelength interval which has a minimum reflectance, and in which an inflection point is made a boundary;
• a step of approximating, by the control device in the first wavelength interval, the reflectance spectrum after being filtered by a high-dimensional function;
• a step of selecting, by the control device, a second wavelength interval having the minimum reflectance from the high-dimensional function;
• a step of approximating, by the control device in the second wavelength interval, the high-dimensional function by a quadratic function, which is lower order than the high-dimensional function;
• a step of differentiating, by the control device, the quadratic function with respect to the wavelength to obtain a solution in which the value thereof becomes zero;
• and
• a step of generating, by the control device from the solution, an output signal indicating a bottom peak wavelength.

A detection device for an intermolecular interaction comprising:

• a detector provided with a ligand;
• a white light source which irradiates white light on the detector;
• a spectrometer which detects reflected light from the detector;
• and
• a control device configured to:
• control the white light source and the spectrometer;
• calculate reflectance over a fixed wavelength interval to obtain a reflectance spectrum;
• approximate the reflectance spectrum by a high-dimensional function;
• select a wavelength interval having the minimum reflectance from the high-dimensional function;
• approximate, in the wavelength interval, the high-dimensional function by a quadratic function, which is lower order than the high-dimensional function;
• differentiate the quadratic function with respect to the wavelength to obtain a solution in which a value thereof becomes zero;
• and
• generate, from the solution, an output signal indicating a bottom peak wavelength.

A detection device for an intermolecular interaction comprising:

• a detector provided with a ligand;
• a white light source which irradiates white light on the detector;
• a spectrometer which detects reflected light from the detector;
• and
• a control device configured to:
• control the white light source and the spectrometer;
• calculate reflectance at a fixed wavelength interval to obtain reflectance spectrum;
• filter the reflectance spectrum;
• select, from the reflectance spectrum after being filtered, a first wavelength interval which has a minimum reflectance, and in which an inflection point is made a boundary;
• approximate, in the first wavelength interval, the reflectance spectrum after being filtered by a high-dimensional function;
• select a second wavelength interval having the minimum reflectance from the high-dimensional function;
• approximate, in the second wavelength interval, the high-dimensional function by a quadratic function, which is lower order than the high-dimensional function;
• differentiate the quadratic function with respect to the wavelength to obtain a solution in which the value thereof becomes zero;
• and
• generate, from the solution, an output signal indicating a bottom peak wavelength.