Hybrid pepper plants resulting from a cross between C. annuum and C. pubescens
AssigneeSeminis Vegetable Seeds, Inc.
InventorsChuong Phan, John Kao, Terry Berke, Carl Jones
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Abstract
The present invention relates to novel methods of producing interspecific hybrids between C. annuum and C. pubescens and progeny thereof. In addition, the present invention provides for the hybrid pepper plants, and parts thereof including their fruit, tissues, and seeds, resulting from a cross between C. annuum and C. pubescens that have nuclear genetic material from both C. annuum and C. pubescens. The hybrid pepper plants of the invention may have a variety of traits including resistance to geminiviruses, tobamoviruses, and resistance to damage by Xanthomonas.
Claims36
A method of producing a fertile interspecific F1 hybrid pepper plant comprising the steps of:
- (a) pollinating a male sterile flower of a Capsicum annuum parent selected from a C. annuum plant or a C. annuum hybrid with pollen from a Capsicum pubescens plant or C. pubescens hybrid to form a pollinated flower;
- (b) treating the pollinated flower with an auxin compound;
- (c) growing the C. annuum parent until the pollinated flower develops into a fruit bearing a seed;
- (d) harvesting the fruit bearing a seed that develops from the pollinated flower;
- and
- (e) rescuing embryonic tissue from the seed of the fruit to produce a fertile interspecific F1 hybrid pepper plant;
- wherein said fertile interspecific F1 hybrid plant has no nuclear genetic material unique to Capsicum eximium and does not have a C. eximium plant as an ancestor.
A method of producing a fertile interspecific F1 hybrid pepper plant comprising the steps of:
- (a) culturing a flower bud of a C. annuum parent selected from a C. annuum plant or C. annuum hybrid to obtain a C. annuum flower;
- (b) pollinating said C. annuum flower with pollen from a C. pubescens plant or a C. pubescens hybrid to form a pollinated flower;
- (c) removing an ovary from said pollinated flower and subculturing said ovary in the presence of an auxin compound and a cytokinin compound;
- (d) maintaining said subcultured ovary until a fruit bearing a seed develops;
- and
- (e) rescuing immature embryo tissue from said seed of said fruit to produce a fertile interspecific F1 hybrid pepper plant;
- wherein said fertile interspecific F1 hybrid plant has no nuclear genetic material unique to Capsicum eximium and does not have a C. eximium plant as an ancestor.
A fertile interspecific hybrid pepper plant, or a part thereof, produced by a method of preparing a hybrid between C. annuum and C. pubescens using a bridging species, said method comprising:
- (a) performing a first cross between a C. annuum plant or a C. annuum hybrid and a plant of a bridging Capsicum species C. chinense to form a C. annuum hybrid;
- (b) crossing one or more progeny from said first cross with a C. pubescens plant or C. pubescens hybrid;
- and
- (c) rescuing embryonic tissue from a seed produced by said crossing in step (b)to form said hybrid between C. annuum and C. pubescens;
- wherein said fertile interspecific hybrid plant has no nuclear genetic material unique to C. eximium and does not have a C. eximium plant as an ancestor.
A fertile interspecific hybrid pepper plant of C. annuum and C. pubescens, or a part thereof, wherein said fertile interspecific hybrid plant has no nuclear genetic material unique to Capsicum eximium or C. cardenasii and does not have a C. eximium or C. cardenasii plant as an ancestor.
A method of preparing a fertile interspecific hybrid between C. annuum and C. pubescens using a bridging species comprising:
- (a) performing a first cross between a C. annuum plant or a C. annuum hybrid and a plant of a bridging Capsicum species C. chinense to form a C. annuum hybrid;
- (b) crossing one or more progeny from said first cross with a C. pubescens plant or C. pubescens hybrid;
- and
- (c) rescuing embryonic tissue from a seed produced by said crossing in step (b) to form said hybrid between C. annuum and C. pubescens;
- wherein said fertile interspecific hybrid plant has no nuclear genetic material unique to C. eximium and does not have a C. eximium plant as an ancestor.
A fertile interspecific hybrid pepper plant, or a part thereof, produced by a method of preparing a hybrid between C. annuum and C. pubescens using a bridging species, said method comprising:
- (a) performing a first cross between a C. pubescens plant or a C. pubescens hybrid and a plant of a bridging Capsicum species C. cardenasii to form a C. pubescens hybrid;
- (b) crossing one or more progeny from said first cross with a C. annuum plant or C. annuum hybrid;
- and
- (c) rescuing embryonic tissue from a seed produced by said crossing in step (b) to form said hybrid between C. annuum and C. pubescens;
- wherein said fertile interspecific hybrid plant has no nuclear genetic material unique to C. eximium and does not have a C. eximium plant as an ancestor.
A method of preparing a fertile interspecific hybrid between C. annuum and C. pubescens using a bridging species comprising:
- (a) performing a first cross between a C. pubescens plant or a C. pubescens hybrid and a plant of a bridging Capsicum species C. cardenasii to form a C. pubescens hybrid;
- (b) crossing one or more progeny from said first cross with a C. annuum plant or C. annuum hybrid;
- and
- (c) rescuing embryonic tissue from a seed produced by said crossing in step (b) to form said hybrid between C. annuum and C. pubescens;
- wherein said fertile interspecific hybrid plant has no nuclear genetic material unique to C. eximium and does not have a C. eximium plant as an ancestor.
Dependencies are inferred from the claim text.