Bacillus thuringiensis gene with lepidopteran activity
AssigneePioneer Hi-Bred International, Inc.
InventorsAndre R. Abad, Hua Dong, Sue B. Lo, Xiaomei Shi, Thomas C. Wolfe
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Abstract
The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
Claims13
A DNA construct comprising a heterologous promoter and a nucleic acid molecule selected from the group consisting of:
- (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1;
- and
- (b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2,
- wherein the heterologous promoter is operably linked to the nucleic acid molecule.
A plant having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having pesticidal activity, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1;
- and
- (b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2;
- wherein said nucleotide sequence is operably linked to a promoter that drives expression of a coding sequence in a plant cell.
A method for protecting a plant from a pest, comprising introducing into said plant or cell thereof at least one expression vector comprising a nucleotide sequence that encodes a pesticidal polypeptide, wherein said nucleotide sequence is selected from the group consisting of:
- (a) a nucleic acid molecule comprising the nucleotide sequence of SEQ ID NO:1;
- and
- (b) a nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:2.
Dependencies are inferred from the claim text.