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US 8,802,927 B2Granted
Stress resistant plants
AssigneeBayer CropScience AG
InventorsMarc De Block, Michael Metzlaff, Veronique Gossele
Life of this patent
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Abstract
Stress tolerance in plants and plant cells is achieved by using nucleotide sequences encoding enzymes involved in the NAD salvage synthesis pathway and/or the NAD de novo synthesis pathway e.g. for overexpression in plants.
Claims14
A method for obtaining a plant with increased stress resistance
- comprising
- introducing a chimeric gene into cells of a plant to obtain transgenic cells, said chimeric gene comprising the following operably linked DNA fragments:
- a plant-expressible promoter;
- a DNA region coding for a nicotinate phosphoribosyltransferase comprising the amino acid sequence of SEQ ID No. 4;
- a 3′ end region involved in transcription termination and polyadenylation;
- regenerating said transgenic cells to obtain a population of transgenic plants;
- and
- selecting a plant from said population of transgenic plants which exhibits increased stress resistance or selecting a plant which exhibits a reduced level of reactive oxygen species or maintains a high level of NADH under stress conditions when compared to a similar non-transgenic plant.
5 more elements
A chimeric gene comprising the following operably linked DNA fragments:
- i. a plant-expressible promoter;
- ii. a DNA region coding for a nicotinate phosphoribosyltransferase comprising the amino acid sequence of SEQ ID No. 4;
- iii. a 3′ end region involved in transcription termination and polyadenylation.
Dependencies are inferred from the claim text.