Method for modifying anthocyanin expression in solanaceous plants
AssigneeNorfolk Plant Sciences Limited
InventorsJie Luo, Eugenio Butelli, Jonathan Jones, Laurence Tomlinson, Catherine Rosemary Martin
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Abstract
The invention provides methods, compositions and transformation systems for modifying the levels of transcription factors in plants such as Solanaceous plants, and introducing disease resistance genes into plants, to produce products with elevated levels of antioxidant compounds, including but not limited to flavonols and chlorogenic acid, as well as exhibiting enhanced disease resistance, and optionally altered appearance. Preferred plants are those in which introduced genes are from Solanaceous species.
Claims29
A method for increasing the level of one of more anthocyanins in a plant of the family Solanaceae or in at least one part of the plant, said method comprising causing or allowing within the plant or in at least one part, the expression of:
- (a) a first heterologous nucleic acid comprising a first nucleotide sequence which encodes Rosea1, Pan1, or a functional variant of Rosea1 comprising at least 90% amino acid sequence identity to SEQ ID NO:
- 84;
- and
- (b) a second heterologous nucleic acid comprising a second nucleotide sequence which encodes Delila or a functional variant comprising at least 90% amino acid sequence identity to SEQ ID NO:
- 86;
- wherein the level of one or more anthocyanins is increased in the plant or in at least one part when compared to the level of one or more anthocyanins in a wild-type plant or part thereof.
A plant or part thereof comprising in its genome:
- (a) a first heterologous nucleic acid comprising a first promoter and an operably linked first nucleotide sequence which encodes Rosea1, Pan1, or a functional variant of Rosea1 comprising at least 90% amino acid sequence identity to SEQ ID NO:
- 84;
- and
- (b) a second heterologous nucleic acid comprising a second promoter and an operably linked second nucleotide sequence which encodes Delila or a functional variant comprising at least 90% amino acid sequence identity to SEQ ID NO:
- 86;
- wherein the level of one of more anthocyanins is increased in the plant or part thereof when compared to the level of the one of more anthocyanins in a wild-type plant or part thereof.
Dependencies are inferred from the claim text.