Engineered landing pads for gene targeting in plants

A method for producing a transgenic plant or plant tissue, the method comprising:

• providing a transgenic plant cell having a transgenic nucleic acid molecule stably integrated into the genome of the plant cell, wherein the transgenic nucleic acid molecule comprises:
• at least two polynucleotides selected from the group consisting of SEQ ID NOs:1-10, and at least one targeting endonuclease recognition site, wherein the at least two polynucleotides selected from the group consisting of SEQ ID NOs:1-10 flank the at least one targeting endonuclease recognition site;
• and
• regenerating the transgenic plant or plant tissue from the transgenic plant cell.

A method for producing a transgenic plant cell, the method comprising:

• providing a transgenic plant cell having a transgenic nucleic acid molecule stably integrated into the genome of the plant cell, wherein the transgenic nucleic acid molecule comprises:
• at least two polynucleotides selected from the group consisting of SEQ ID NOs:
• 1-10, and at least one targeting endonuclease recognition site, wherein the at least two polynucleotides selected from the group consisting of SEQ ID NOs:1-10 flank the at least one targeting endonuclease recognition site;
• providing at least one targeting endonuclease or a first nucleic acid molecule comprising a nucleic acid sequence encoding the at least one targeting endonuclease, wherein the targeting endonuclease(s) recognize targeting endonuclease recognition site(s);
• providing a second nucleic acid molecule comprising a nucleic acid sequence of interest and two additional nucleic acid sequences flanking the nucleic acid sequence of interest, wherein each of the two additional nucleic acid sequences is homologous with one of the polynucleotides selected from the group consisting of SEQ ID NOs:
• 1-10;
• and
• introducing into the plant cell the at least one targeting endonuclease or first nucleic acid molecule, and the second nucleic acid molecule, so as to stably integrate the nucleic acid sequence of interest via homology-driven integration into the genome of the plant cell.