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AssigneeSanaria Inc.
InventorsStephen L. Hoffman, Thomas C. Luke
Estimating the term…
Disclosed are apparatuses and methods for the production of attenuated aseptic parasites in haematophagous insects generally, and production of Plasmodium species sporozoites in Anopheles species mosquitoes specifically; apparatuses and methods for the production of strains of haematophagous insects with desired properties such as hypoallergenicity or hyperinfectivity; methods of producing a parasite strain that is capable of withstanding cyropreservation at temperatures close to freezing; apparatuses and methods for the injection of an attenuated parasite vaccine; production of parasites and haematophagous insects that are free from contamination by unwanted biological agents; apparatuses for the reconstruction of complex parasitic life cycles aseptically to avoid the contamination of the parasite or the insect vector host with unwanted biological agents.
Aseptic Anopheles-species mosquitoes, said mosquitoes grown from sterilized Anopheles-species eggs in a mosquito rearing chamber with solid walls and airtight seals, said walls enclosing a sterilized interior space and adapted for filtered air passage, and said chamber provided with aseptic larval rearing broth;
Metabolically active aseptic sporozoite-stage Plasmodium-species parasites selected from the group consisting of P. falciparum, P. vivax, P. ovale, and P. malariae, wherein said metabolically active sporozoite-stage parasites are isolated from aseptic Anopheles-species mosquitoes infected by feeding fed on a sterile blood meal comprising infectious gametocyte-stage parasites;
Aseptic Anopheles mosquitoes infected with metabolically active aspetic sporozoite-stage Plasmodium-species parasites selected from the group consisting of P. falciparum, P. vivax, P. ovale, and P. malariae, wherein said infection results from the aseptic mosquitoes feeding on a sterile blood meal comprising infectious gametocyte-stage parasites;
Dependencies are inferred from the claim text.