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US 8,802,838 B2Granted
Ultra high-throughput opti-nanopore DNA readout platform
AssigneePresident and Fellows of Harvard College
InventorsAmit Meller, Jerome Mathe, John S. Eid
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Abstract
Described herein are methods for analyzing polymer molecules. These methods are employed for the high throughput readout of DNA and RNA molecules with single molecule sensitivity. The method of the present invention comprises (1) the electrically controlled unzipping of DNA (or RNA) double strands, and (2) the readout of the molecule's identity (or code) using one or more molecule signal detection.
Claims26
A composition comprising:
- a polynucleotide polymer comprising a plurality of oligonucleotides joined together in a sequence corresponding to the nucleotide sequence of a target polynucleotide, wherein one or more oligonucleotides in the polynucleotide polymer correspond to one or more predetermined nucleotide bases of the target polynucleotide;
- a plurality of quenchable fluorescent probes, each quenchable fluorescent probe comprising a nucleotide sequence, a fluorophore and a quencher, wherein:
- each quenchable fluorescent probe is only hybridized to one of the oligonucleotides in the polynucleotide polymer;
- and
- each of the fluorophores, except a fluorophore of one of the terminal quenchable fluorescent probes, is quenched by a quencher of an adjacent quenchable fluorescent probe.
2 more elements
Dependencies are inferred from the claim text.