Cloning and recombinant productions of Vespula venom protease and methods of use thereof
AssigneePLS-Design GmbH
InventorsHenning Seismann, Ingke Braren, Thomas Grunwald
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Abstract
The invention relates to nucleic acids encoding a novel Vespula venom protease or fragments thereof, in particular the protease Ves v 4, recombinant vectors comprising such nucleic acids, and host cells containing the recombinant vectors. The invention is further directed to the expression of such nucleic acids to produce a recombinant Vespula venom protease, or recombinant fragments thereof, or synthetic peptides thereof. Such a protease or fragments thereof or synthetic peptides thereof are useful for diagnosis of insect venom allergy and for therapeutic treatment of insect venom allergy.
Claims8
A cDNA molecule having a nucleic acid sequence selected from the group consisting of:
- i) the nucleotide sequence as shown in SEQ ID NO:1;
- and
- ii) a nucleic acid sequence that is at least 90% identical to the nucleic acid sequence as shown in SEQ ID NO:1 and encodes a proteolytically active polypeptide.
A nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of:
- i) the cDNA nucleotide sequence as shown in SEQ ID NO:1;
- and
- ii) a nucleic acid sequence that is at least 90% identical to the cDNA nucleotide sequence as shown in SEQ ID NO:1 and that encodes a proteolytically active polypeptide,
- wherein said nucleic acid sequence is operationally associated with transcription and/or translation elements suitable for expression in a host cell, or in a cell-free translation system.
Dependencies are inferred from the claim text.